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Dissecting cross-reactivity in polyclonal IgG1 responses against SARS-CoV-2 variants of concern

ABSTRACT: Using a mass spectrometry-based approach we monitored individual donors IgG1 clonal responses following a SARS-CoV-2 infection. We monitored the plasma clonal IgG1 profiles of 8 donors who had experienced an infection by either the wild type Wuhan Hu-1 virus or one of 3 VOCs (Alpha, Beta and Gamma). In these donors we charted the full plasma IgG1 repertoires as well as the IgG1 repertoires targeting the SARS-CoV-2 spike protein trimer VOC antigens. The plasma of each donor contained numerous anti-Spike IgG1 antibodies, accounting for <0.1% up to almost 10% of all IgG1s. Some of these antibodies were observed to be VOC-specific whereas others did recognize multiple or even all VOCs. We show that in these polyclonal responses, each clone exhibits a distinct cross-reactivity and also distinct virus neutralization capacity. These observations support the need for a more personalized look at the pallet of antibody clonal responses to infectious diseases.

INSTRUMENT(S): Orbitrap Exploris 480

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Albert J.R. Heck

PROVIDER: MSV000089833 | MassIVE | Thu Jul 07 03:17:00 BST 2022

REPOSITORIES: MassIVE

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