DSSO Crosslinking of Intact Tetrahymena Thermophila Cilia

ABSTRACT: The MS-cleavable crosslinker, DSSO, was added to cilia isolated from Tetrahymena thermophila. After digestion crosslinked peptides were enriched by size exclusion chromatography. Data were collected using an MS2/MS3 method. Analysis was done using the XlinkX node of PD2.3 and a fasta file generated from standard MS1/MS2 analysis of the crosslinked samples.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Tetrahymena Thermophila (ncbitaxon:5911)

SUBMITTER: Edward Marcotte  

PROVIDER: MSV000089917 | MassIVE | Tue Jul 19 11:32:00 BST 2022

SECONDARY ACCESSION(S): PXD035387

REPOSITORIES: MassIVE