Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Proteomic analysis of yeast mutants treated with rapamycin

ABSTRACT: 7 S. cerevisiae loss-of-function mutants were treated with either the drug rapamycin or a control for 30 minutes. Protein extracts were generated and the resulting peptides were subjected to tryptic digestion and bottom up, data independent proteome analysis.

INSTRUMENT(S): Q Exactive HF-X

ORGANISM(S): Saccharomyces Cerevisiae (ncbitaxon:4932)

SUBMITTER: Duncan Holbrook-Smith

PROVIDER: MSV000089935 | MassIVE | Thu Jul 21 08:19:00 BST 2022

REPOSITORIES: MassIVE

ACCESS DATA

Json Xml

Similar Datasets

Proteomic analysis of a CFF1 deletion mutant under rapamycin treatment

Project description:Yeast harbouring a CFF1 deletion as well as wild-type S. cerevisiae were treated with either the drug rapamycin or a control for 30 minutes. Protein extracts were generated and the resulting peptides were subjected to tryptic digestion and bottom up, data independent proteome analysis.

2022-07-21 | MSV000089940 | MassIVE

Transcription profiling by array of yeast ARO80, DAT1 and RTG3 deletion mutants after rapamycin perturbation

Project description:We profiled the expression levels of three single deletion mutants (ARO80, DAT1, RTG3) of the the wild type strain BY4742 50 minutes after rapamycin perturbation. Our goal is to validate regulatory networks inferred from microarray data E-MTAB-412.

2011-09-30 | E-MTAB-446 | biostudies-arrayexpress

Transcription profiling of yeast treated with rapamycin in a 7-part time series

Project description:This experiment was a 7-part time series (or, 2x7 factorial design) examining the effect of rapamycin on gene expression in Saccharomyces cerevisiae. Treated and untreated cultures were compared at 0, 20, 40, 60, 120, 240, and 360 minutes. In the paper, expression ratios (mRNA) at each time point were compared to equivalent ratios (protein) from shotgun proteomics NSAF data. High-expressing mRNA ratios were also clustered and GO-characterized.

2010-02-05 | E-TABM-639 | biostudies-arrayexpress

Transcription profiling of rapamycin treated wild type and ume1-deletion yeast

Project description:Rapamycin treatment of wild type and ume1-delta yeast S. cerevisiae strain.

2008-09-04 | E-MEXP-1782 | biostudies-arrayexpress

High-throughput metabolome profiling of the response of 164 yeast mutants to rapamycin

Project description:164 yeast loss-of-function mutant strains were exposed to the drug rapamycin for 0, 5, 30, 60 and 90 minutes. Intracellular metabolite extracts were prepared at each time point, and those extracts were measured by flow-injection analysis time-of-flight mass spectrometry.

2022-07-21 | MSV000089939 | MassIVE

Yeast AlaRS mutants

Project description:RNA sequencing of Saccharomyces cerevisiae wild type and alanyl-tRNA synthetase mutants grown at 30 degrees and challenged at 37 degrees

2021-06-23 | GSE178694 | GEO

Transcription profiling of yeast treated with rapamycin

Project description:A diploid yeast strain was grown at 30C in YPD medium. At an OD of 0.6 (600nm) rapamycin was added to a final concentration of 0.20 ug/ml and the incubation continued for 90 min. A control culture was treated with an equal amount of DMSO vehicle. Cells were harvested at an OD of 0.9 rapamycin and 1.1 control.

2003-11-09 | E-MANP-2 | biostudies-arrayexpress

Expression data of yeast genes treated with rapamycin and compound CID 3528206

Project description:Compound CID 3538206 inhibits yeast TORC1 activity and functionally mimic rapamycin. We used microarrays to compare the global gene expression with the treatment of CID 3528206 and rapamycin.

2012-05-01 | GSE33320 | GEO

Expression analysis of wild-type and transcription factor mutants in response to rapamycin

Project description:Transcript level changes in transcription factor mutants after rapamycin treatment, compared to the wild-type strain. This data set accompanies the study "Widespread Misinterpretable ChIP-seq Bias in Yeast" (GSE51251)

2013-11-15 | GSE51376 | GEO

Transcriptional profiling of Rapamycin-treated ptc6 and ptc1ptc6 mutant yeast cells

Project description:We are interested in the study of the sensitivity of yeast cells lacking either the type 1 protein phosphatase Ptc6 or Ptc1 and Ptc6 to the drug rapamycin. In order to carry out our studies, we analyzed the changes in the transcription profile that a short-term incubation with rapamycin (200 ng/mL) has in wild type, ptc6 mutant and ptc1 ptc6 double mutant cells. It has been shown that inhibition of the TOR pathway by treatment with rapamycin has profound effects on the global transcriptional profile. We confirmed the previously described transcription changes induced by the drug in wild type cells and characterized an notable attenuation of the transcriptional response in cells lacking either PTC6 or PTC1 and PTC6 genes.

2013-04-19 | GSE38256 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data