Project description:Four dye swap replicates were performed with material derived 24 hours after stimulation of HUVECs with PMA and RMAC11 hybridised to material obtained at time 0 hours. The cDNAs used in this microarray were identified as part of collaborative project between the IMVS and Bionomics Limited. The project aims were to identify genes up-regulated during in vitro capillary tube formation as targets for angiogenesis-based therapeutics. Any requests for further information regarding the data generated from this collaboration should be directed to Bionomics Limited (www.bionomics.com.au) at the following address: Bionomics Limited 31 Dalgleish Street Thebarton, South Australia Australia, 5031 Phone: 618 8354 6104 Fax: 618 8354 6199 Email: busdev@bionomics.com.au Keywords = angiogenesis Keywords = HUVEC Keywords = in vitro Keywords = capillary tube Keywords: repeat sample
Project description:In order to detect changes in liver tissue protein translation efficiency during liver regeneration, we performed sucrose density gradient centrifugation to separate translated (associated with polysome fraction), and non-translated (associated with sub-polysome fraction) transcripts in extracts from mouse livers 0 h and 24 h post-surgery. We found that compared to liver tissues before surgery, extracts from tissues 24 h post-surgery had significantly fewer transcripts associated with polysomes, suggesting a reduction in protein synthesis activity.
Project description:Transcriptional profiling of A. nidulans comparing starvation for 0 (reference), 12 and 24 h. The main objective was to identify genes specifically regulated during starvation by atmA and xprG. The results of the experiment were further validated by real-time PCR.
Project description:K562 cells (duplicate cultures A & B) are treated with 50 micromolar hemin for 0, 6, 12, 24, 48, 72 hours followed by RNA extraction and gene expression profiling on Affymetrix human U133A arrays and analysis by MAS 5.0 Keywords: other