Dataset Information

Vibrio cholerae secDF1 whole cell proteomics

ABSTRACT: Whole cell pellets of Vibrio cholerae strains as described in Sit et al, 2022 (https://www.biorxiv.org/content/10.1101/2022.02.04.479082v1) Proteomics performed by the Thermo Center for Multiplexed Proteomics at Harvard Medical School using a standard fractionated total proteomics workflow. Each RAW file corresponds to one analyzed fraction out of twelve. Mapping of samples to tags is listed in the Supplementary File attached to this submission. BS001: WT V. cholerae (four replicates) BS553: delta secDF1 V. cholerae (three replicates). BS558, BS280, and BS338 correspond to strains analyzed in the same run but not presented in the manuscript.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Vibrio Cholerae

SUBMITTER: Matthew Waldor

PROVIDER: MSV000090217 | MassIVE | Fri Aug 26 09:26:00 BST 2022

REPOSITORIES: MassIVE

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Publications

Undecaprenyl phosphate translocases confer conditional microbial fitness.

Sit Brandon B Srisuknimit Veerasak V Bueno Emilio E Zingl Franz G FG Hullahalli Karthik K Cava Felipe F Waldor Matthew K MK

Nature 20221130 7945

The microbial cell wall is essential for maintenance of cell shape and resistance to external stressors1. The primary structural component of the cell wall is peptidoglycan, a glycopolymer with peptide crosslinks located outside of the cell membrane1. Peptidoglycan biosynthesis and structure are responsive to shifting environmental conditions such as pH and salinity2-6, but the mechanisms underlying such adaptations are incompletely understood. Precursors of pept ...[more]

PMID: 36450355

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Project description:The PFGRC has developed a cost effective alternative to complete genome sequencing in order to study the genetic differences between closely related species and/or strains. The comparative genomics approach combines Gene Discovery (GD) and Comparative Genomic Hybridization (CGH) techniques, resulting in the design and production of species microarrays that represent the diversity of a species beyond just the sequenced reference strain(s) used in the initial microarray design. These species arrays may then be used to interrogate hundreds of closely related strains in order to further unravel their evolutionary relationships. Many infectious agents that cause emerging and re-emerging diseases appear to evolve from non-virulent forms. We still lack a clear understanding about the natural history of various microbial agents that cause human infectious diseases and the events leading to acquisition of their pathogenic potential. There have been seven pandemics of V. cholerae throughout the history of the mankind. To date, the world population is still experiencing the seventh one which started in the early 1960s. From almost 200 recognized V. cholerae serotypes, the majority of these epidemics are associated with primarily O1 serotype. However there is evidence that this species is undergoing some phenotypic changes during the last decades. Such examples include shifts in some metabolic pathways used for biotyping, phage sensitivity profiling and the acquisition of plasmids that carry multiple genes conferring antimicrobial resistance. Furthermore, the recent emergence of a non-O1 serotype (‘Bengal strain’, classified serologically as O139) has prompted the experts to think that perhaps this genotype will be the predominant one in the upcoming (eighth) pandemic. Besides the O1 and O139, the non-O1 and non-O139 V. cholerae stains are occasionally associated with other severe forms of gastrointestinal disease in humans. Interestingly, many of these non-canonical strains lack the genes encoding the typical virulence factors for this species such as the Cholera-toxin (ctx) and toxin co-regulated pilus (tcpA). Therefore it has been hypothesized that this group of non-canonical V. cholerae pathogens consist of several sub-clones that elicit disease via unknown virulence determinants and underlying mechanisms. The flow of genetic information within this group motivated us to identify novel genes for the purpose of creating a "species" DNA microarray to better understand the ancestral relationships among its members. Based on preliminary genotyping (MLST, and CGH using a single-genome-based array), 10 diverse V. cholerae and one V. mimicus were selected for sequencing. Sequence information obtained from this project, and from other publicly available sources, led to the development of a comprehensive species microarray for V. cholerae group members. The availability of the V. cholerae species DNA microarray has allowed us to carry out a collaborative CGH genotyping project to validate this microarray as well as understand the phylogenomic relationships among members of V. cholerae group.

Dataset Information

Vibrio cholerae secDF1 whole cell proteomics

Publications

Undecaprenyl phosphate translocases confer conditional microbial fitness.

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