Dataset Information

Chen Minor et al 2023 Mass Spectrometry Data

ABSTRACT: Purified protein complexes were separated by SDS-PAGE and gel lanes divided into 3 sections (A, B and C). Samples prepared in triplicate (1, 2 and 3). A nanoElute (Bruker) was attached in line to a timsTOF Pro equipped with a CaptiveSpray Source (Bruker, Hamburg, Germany). Chromatography was conducted at 40C through a 25cm reversed-phase C18 column (PepSep) at a constant flow-rate of 0.5 uL/min. Mobile phase A was 98/2/0.1% LC/MS grade H2O/ACN/FA (v/v/v) and phase B was LC/MS grade ACN with 0.1% FA (v/v). During a 108 min method, peptides were separated by a 3-step linear gradient (5% to 30% B over 90 min, 30% to 35% B over 10 min, 35% to 95% B over 4 min) followed by a 4 min isocratic flush at 95% for 4 min before washing and a return to low organic conditions. Experiments were run as data-dependent acquisitions with ion mobility activated in PASEF mode. MS and MS/MS spectra were collected with m/z 100 to 1700 and ions with z = +1 were excluded.

INSTRUMENT(S): timsTOF

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Balyn Zaro