MALDI informed microdissected airway proteomics

ABSTRACT: After performing MALDI metabolomics analysis, we identified airway regions. On a consecutive slide we used laser capture microdissection (LCM) to cut out regions of interest that had an airway specific signal by MALDI. The samples were prepped using the MicroPOTS protocol (PMID: 33491460) and analyzed by LC-MS/MS. The search was performed using MaxQuant and both LFQ and iBAQ quantification were performed.

INSTRUMENT(S): Q Exactive Plus

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Geremy Clair  

PROVIDER: MSV000090561 | MassIVE | Thu Oct 20 15:59:00 BST 2022

REPOSITORIES: MassIVE