Multimodal measurement of the transcriptome and proteome in G2/M arrested single cells using nanoSPLITS

ABSTRACT: Single-cell proteomic data from RO-3306 treated alveolar type II epithelial cells (C10) and untreated C10 cells. Cells were sorted using cellenONE onto several nanoSPLITS chips. Cell lysates were then split for parallel RNAseq and proteomic analysis. For proteomics recovery experiment where both chips were analyzed by proteomics, 10 C10 cells were pooled onto a single cell before splitting. For the proteomic sample prep all steps utilized the nanoPOTS sample handling robot. Lysates were reduced, treated with IAA, digested overnight with trypsin/Lys-C at 37C, and vacuum dried / stored at -20C. LC-MS/MS data was acquired using TIFF DDA methodology on a Thermo Tribrid mass spectrometer. Data was searched using FragPipe version 18.0 before downstream analysis in R.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Mus Musculus (ncbitaxon:10090)

SUBMITTER: Ying Zhu  

PROVIDER: MSV000090828 | MassIVE | Fri Dec 02 22:43:00 GMT 2022

REPOSITORIES: MassIVE