Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Immunoprecipitation was performed with HCT116 cells overexpressing 3xFLAG-SET/TAF-Ib with anti-FLAG antibodies

ABSTRACT: Immunoprecipitation was performed with HCT116 cells overexpressing 3xFLAG-SET/TAF-Ib with anti-FLAG antibodies. The immunoprecitates were separated by SDS-PAGE and putative interaction partners of SET/TAF-Ib were identified with mass spectrometry.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Sang Beom Seo

PROVIDER: MSV000090898 | MassIVE | Tue Dec 13 19:51:00 GMT 2022

SECONDARY ACCESSION(S): PXD038806

REPOSITORIES: MassIVE

ACCESS DATA

Json Xml

Similar Datasets

Expression data from hela cells stable clones overexpressing TFEB-3xFLAG

Project description:In order to identify the effects of TFEB overexpression on the hela cells transcriptome, we performed Affymetrix Gene-Chip hybridization experiments for the hela TFEB stable clones Transcriptome analysis of hela stable clones overexpressing TFEB-3xFLAG

2021-07-06 | GSE73054 | GEO

Global mRNA expression profiling of HCT116 cells overexpressing miR30a-5p

Project description:Global mRNA expression profiling of HCT116 cells overexpressing miR30a-5p were collected using Agilent human whole genome array (G4845A AMADID 026652, cRNA 4x44k V2) . Two different sources of RNA were analyzed: 1.) HCT116 colon cancer cells overexpressing human mir-30a-5p together with a sponge vector expressing binding sites for the antisense sequence of sh30a-5p vector construct to compete out possible miRNAs derived from the anti-guide strand of this vector. 2.) HCT116 colon cancer cells expressing control vector (pLKO.1-LV) and the sponge vector described above.

2012-03-08 | GSE29921 | GEO

Global mRNA expression profiling of HCT116 cells overexpressing miR30a-5p

2012-03-08 | E-GEOD-29921 | biostudies-arrayexpress

CAR-macrophages for the Treatment of HER2 Overexpressing Solid Tumors

Project description:Phase 1, first-in-human, open label study of CAR macrophages in HER2 overexpressing solid tumors.

| 2360169 | ecrin-mdr-crc

ChIP-Seq with anti-FLAG antibodies to determine binding of LexA-3xFLAG to DNA in Streptomyces venezuelae treated with Mitomycin C (MMC) overnight

Project description:LexA is a transcriptional repressor for genes requiring expression primarily during SOS response in response to stress such as that caused by DNA alkylating agent Mitomycin C (MMC). LexA undergoes self-cleavage under stress conditions thereby lifting the repression on genes whose expression is required for stress response. In our experiment LexA binding sites in the genome were determined in untreated and MMC treated (overnight, 14h) cultures using a LexA-3xFLAG strain and anti-FLAG monoclonal antibodies. Wild type Streptomyces venezuelae (unFLAGged LexA) cultures, with and without MMC treatment were used as negative controls.

2022-07-06 | E-MTAB-11603 | biostudies-arrayexpress

ChIP-seq after immunoprecipitation with either IgG or SOX2 antibodies in murine genetically modified stem cells overexpressing the RAX gene (CCE-Rx cells)

Project description:In order to identify target genes of the SOX2 transcription factor, we tried to identify SOX2 binding site in murine genetically modified stem cells overexpressing the RAX gene (CCE-Rx cells) by ChIP-seq. SOX2 gene mutations are involved in anophthalmia and our hypothesis was that target genes of SOX2 transcription factor may also be candidate to be involved in ocular development.

2015-11-04 | GSE74599 | GEO

RIP-seq of MSTR-1::FLAG and N2

Project description:Worms expressing 3xFLAG-tagged MSTR-1 (F22D6.2) were grown to young adult stage, crosslinked with 2% formaldehyde, and RIP-seq was performed to identify binding regions on target RNAs

2023-10-01 | GSE185303 | GEO

Chromatin immunoprecipitation of yeast using anti-H3C and anti-K56 antibodies

Project description:Chromatin immunoprecipitation DNA by H3 K56 acetylation antibody

2007-09-28 | E-MEXP-1248 | biostudies-arrayexpress

iCLIP of FLAG-PURA overexpression with anti-FLAG antibody in HeLa cells

Project description:The protein PURA binds to DNA and RNA and has been suggested to be implicated in different cellular functions. Here, we show that PURA predominantly resides in the cytoplasm, where it binds to a large set of transcripts.

2022-12-12 | GSE193903 | GEO

Chromatin Immunoprecipitation of UV treated AdHBx cells with anti-HBx antibodies

Project description:Direct gene targets of HepG2 cells transduced with Adenoviral vector carrying HBx vs Adenoviral vector under UV treatment Keywords: ChIP-chip profiling

2008-07-01 | GSE11106 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data