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Bottom-up Proteomics Analysis for Adduction of the Broad Spectrum Herbicide Atrazine to Histone

ABSTRACT: Whole histones of calf thymus or human histone H3.3 was incubated with atrazine. After solvent-based protein precipitation, the protein was digested by trypsin/Glu-C and the resulting peptides were analyzed by LC-MS/MS. The resulting tryptic/Glu-C peptide of DTNLCAIHAK from calf thymus or human H3.3 was identified with an accurate mass shift of +179.117 Da in atrazine incubated samples. It is deduced that a chemical group with elemental composition of C8H13N5 (179.1171 Da) from atrazine adducted with calf thymus or human histone H3.3. It was confirmed by MS/MS analysis that the adduction position was at its cysteine110 residue.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Bos Taurus (ncbitaxon:9913) Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Shaogang Chu Robert J. Letcher

PROVIDER: MSV000090917 | MassIVE | Fri Dec 16 10:59:00 GMT 2022

REPOSITORIES: MassIVE

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