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Obtaining Increased Functional Proteomics Insights from Thermal Proteome Profiling Experiments through Optimized Melt Shift Calculation and Statistical Analysis

ABSTRACT: HEK293A cells treated with either DMSO or 1 micromolar Thapsigargin for 1 hour. Lysates were heat treated at 8 temperatures and supernatant was reduced, alkylated and digested with Trp/LysC. Samples were desalted and labeled with TMTPro.

INSTRUMENT(S): Orbitrap Fusion Lumos, Orbitrap Exploris 480

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Amber L. Mosley

PROVIDER: MSV000090932 | MassIVE | Mon Dec 19 16:01:00 GMT 2022

SECONDARY ACCESSION(S): PXD044418

REPOSITORIES: MassIVE

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