Optimum collision energies for proteomics: The impact of ion mobility separation
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ABSTRACT: We performed LC-MS/MS measurements on a Waters Select Series Cyclic IMS QTof mass spectrometer with varied CE settings both with and without IMS. We investigated the CE dependence of identification score, using Byonic search engine, for more than 1000 tryptic peptides from HeLa digest standard. We determined the optimal CE values, giving the highest identification score, for both setups (i.e., with and without IMS). Further, the two CID fragmentation cell of the instrument, located before and after the IMS cell, were also compared.
INSTRUMENT(S): SELECT SERIES Cyclic IMS
ORGANISM(S): Homo Sapiens (ncbitaxon:9606)
SUBMITTER: Agnes Revesz
PROVIDER: MSV000090944 | MassIVE | Tue Dec 20 20:37:00 GMT 2022
REPOSITORIES: MassIVE
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