Dataset Information

Circadian rhythm disruption is associated with skeletal muscle dysfunction within the blind Mexican Cavefish

ABSTRACT: Circadian rhythm disruption is associated with skeletal muscle dysfunction within the blind Mexican Cavefish

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Astyanax Mexicanus (ncbitaxon:7994)

SUBMITTER: Laurence Florens

PROVIDER: MSV000091062 | MassIVE | Fri Jan 13 11:23:00 GMT 2023

SECONDARY ACCESSION(S): PXD039429

REPOSITORIES: MassIVE

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Publications

Circadian rhythm disruption linked to skeletal muscle dysfunction in the Mexican Cavefish.

Olsen Luke L Krishnan Jaya J Banks Charles C Hassan Huzaifa H Rohner Nicolas N

Current biology : CB 20230401 7

It has become clear that circadian clocks in peripheral tissues play important functions. Disruption of the circadian clock in skeletal muscle, for example, results in insulin resistance, sarcomere disorganization, and muscle weakness1. Interestingly, cavefish, which exhibit a disrupted central clock, exhibit similar muscle phenotypes2,3,4, raising the question of whether they are caused by alterations to central or peripheral clo ...[more]

PMID: 37040703

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Project description:Disruption of the Circadian Clock within the Cardiomyocyte Influences Myocardial Contractie Function, Metabolism, and Gene Expression Virtually every mammalian cell, including cardiomyocytes, possesses an intrinsic circadian clock. The role of this transcriptionally-based molecular mechanism in cardiovascular biology is poorly understood. We hypothesized that the circadian clock within the cardiomyocyte influences diurnal variations in myocardial biology. We therefore generated a cardiomyocyte-specific circadian clock mutant (CCM) mouse, in order to test this hypothesis. At 12 weeks of age, CCM mice exhibit normal myocardial contractile function in vivo, as assessed by echocardiography. Radiotelemetry studies reveal attenuation of heart rate diurnal variations and bradycardia in CCM mice (in the absence of conduction system abnormalities). Reduced heart rate persisted in CCM hearts perfused ex vivo in the working mode, highlighting the intrinsic nature of this phenotype. Wild-type, but not CCM, hearts exhibited a marked diurnal variation in responsiveness to an elevation in workload (80mmHg plus 1 microM epinephrine) ex vivo, with a greater increase in cardiac power and efficiency during the dark (active) phase versus the light (inactive) phase. Moreover, myocardial oxygen consumption and fatty acid oxidation rates were increased, while cardiac efficiency was decreased, in CCM hearts. These observations were associated with no alterations in mitochondrial content or structure, and modest mitochondrial dysfunction, in CCM hearts. Gene expression microarray analysis identified 548 and 176 genes in atria and ventricles, respectively, whose normal diurnal expression patterns were altered in CCM mice. These studies suggest that the cardiomyocyte circadian clock influences myocardial contractile function, metabolism, and gene expression. Keywords: Comparison of circadian oscillations in gene expression in hearts taken from wildtype and transgenic animals

Dataset Information

Circadian rhythm disruption is associated with skeletal muscle dysfunction within the blind Mexican Cavefish

Publications

Circadian rhythm disruption linked to skeletal muscle dysfunction in the Mexican Cavefish.

Similar Datasets

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