Systemic toxicity of snake venom metalloproteinases: proteomics analyses of kidney injury and blood plasma disturbances in a mouse model

ABSTRACT: Metalloproteinases are abundant in viperid venoms and are implicated in several envenomation effects. Bothrops jararaca venom is reported to induce kidney injury and coagulopathy. HF3 is an extremely hemorrhagic metalloproteinase from B. jararaca venom and participates in the local pathogenesis of envenomation. Here we applied proteomics approaches to evaluate the effects of HF3 in the mouse kidney and blood plasma after 2 h and 6 h of HF3 injection in the thigh muscle. All proteomic analyses were performed using orbitrap-based high-resolution mass spectrometry. For kidney tissue and plasma proteomic analysis, proteins were extracted and submitted to trypsin digestion and peptides were TMT-labeled for relative quantification. For N-terminomic analysis of kidney tissue, a modified Terminal Amine Isotopic Labeling of Substrates (TAILS) protocol was applied, using TMT-tags for free-amine blocking and relative quantification.

INSTRUMENT(S): Q Exactive Plus

ORGANISM(S): Mus Musculus (ncbitaxon:10090)

SUBMITTER: Prof. Oliver Schilling  

PROVIDER: MSV000091101 | MassIVE | Fri Jan 20 01:18:00 GMT 2023

SECONDARY ACCESSION(S): PXD039562

REPOSITORIES: MassIVE