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Modulation of GPR133 (ADGRD1) Signaling by its Intracellular Interaction Partner Extended Synaptotagmin 1 (ESYT1)

ABSTRACT: GPR133 (ADGRD1) is an adhesion G protein-coupled receptor that signals through G alpha's and is required for growth of glioblastoma (GBM), an aggressive brain malignancy. The regulation of GPR133 signaling is incompletely understood. Here, we use proximity biotinylation-mass spectrometry to identify ESYT1, a Ca2+-dependent mediator of endoplasmic reticulum-plasma membrane bridge formation, as an intracellular interactor of GPR133. ESYT1 knockdown or knockout increases GPR133 signaling, while its overexpression has the opposite effect, without altering GPR133 levels in the plasma membrane.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Dr. Thomas A. Neubert Dr. Dimitris G. Placantonakis

PROVIDER: MSV000091163 | MassIVE | Thu Jan 26 13:56:00 GMT 2023

REPOSITORIES: MassIVE

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