Raw mas spectra of bacterial proteins

ABSTRACT: Bovine mastitis is the most common disease that affects dairy cattle worldwide and generates millions of losses for cattle breeders. One of the most common pathogens identified in infected milk samples is Staphylococcus aureus. Presently, there is no fast, bacterial species-recognizing test in the diagnostic market. The aim of this study was bioinformatic detection and characteristic of fibronectin binding protein A (FnBPA) of Staphylococcus aureus (SA) and investigation of this protein in milk samples obtained from cows with diagnosed mastitis. Bioinformatic detection of potential biomarker for bovine SA obtained analyses of >90.000.000 amino acid sequences. Analyses on FnBPA included, among others: detection of signal peptides, non-classical proteins, antigenicity, and epitopes prediction. To confirm a presence of the fnbA gene in four SA isolates amplification with specific primers was performed. Detection of FnBPA was carried out by immunoblotting. Proteins mixture was separated by SDS-PAGE, and immunoreactivity and selectivity were performed by monoclonal anti-FnBPA antibodies and SA-negative serum. Bioinformatic analyses showed that FnBPA is a surface, conservative, immunoreactive, and species-specific protein with antigenic potential. Molecular methods confirmed its presence in 100% of isolates, and immunoblotting proved its immunoreactivity and specificity. Thus, it can be considered as potential biomarker in immunodiagnostic of mastitis.

INSTRUMENT(S): ultraflex III TOF/TOF

ORGANISM(S): Bacteria (ncbitaxon:2)

SUBMITTER: Dorota Pietras-Ozga   Katarzyna Michalak   Anna Dobrut  

PROVIDER: MSV000091181 | MassIVE | Thu Jan 26 13:56:00 GMT 2023

REPOSITORIES: MassIVE