20230301_LCICPMS_BaarsLab_Scopulariopsis

ABSTRACT: LC separation was performed on a Thermo Dionex Ultimate 3000 UPLC system using an ACE excel 1.7 super C18 2.1 x 100 mm UPLC column with a flow rate of 0.35 mL/min. Solvent A was MilliQ water with 5 mM ammonium formate adjusted to pH 6.5 using ammonium hydroxide. Solvent B was 95:5 ACN:MilliQ water with 5 mM ammonium formate adjusted to pH 6.5 using ammonium hydroxide. Solvent was held at 0% B for 2 minutes and then a gradient of 0 - 100% B over 15 minutes was run and brought back down to 0% B at 15.2 minutes. For each sample, 20 uL of a 1 mg/mL solution was injected. The LC flow was connected to a computer-controlled Thermo iCAP RQ ICPMS controlled by QTEGRA and operating in LC mode for ICP-MS data collection. Additional oxygen gas flow to the inlet was set to 4.50%, cool gas 14.00 L/min, auxiliary gas 0.80 L/min, plasma power 1550.0 W. Plasma exhaust was 0.34 mbar and plasma cooling water flow was 0.68 L/min. The Q-Cell collision gas was 4.708 mL/min. The isotopes chosen for analysis were 48Ti, 55Mn, 57Fe, 56Fe, 59Co, 60Ni, 63Cu, 64Zn, 65Cu, 66Zn, and 95Mo, because these transition metals have been found chelated to microbial ionophores.

INSTRUMENT(S): LC-ICPMS

ORGANISM(S): Scopulariopsis Sp. Jb370

SUBMITTER: Oliver Baars  

PROVIDER: MSV000091384 | MassIVE | Wed Mar 01 11:33:00 GMT 2023

REPOSITORIES: MassIVE