Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

SILAC Experiment for A549 cells treated with UV and 2-deoxyglucose

ABSTRACT: SILAC labelled peptides from control and UV or 2-deoxy glucose treated A549 cells. Control is light labelled and treatment groups are heavy labelled. Fractions are as per below: UV1: APM-0331-2 to APM-0331-6 UV2: APM-0331-12 to APM-0331-16 2DG1: APM-0331-7 to APM-0331-11 2DG2: APM-0331-17 to APM-0331-21

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Evangelos Michelakis

PROVIDER: MSV000091503 | MassIVE | Thu Mar 16 15:21:00 GMT 2023

REPOSITORIES: MassIVE

ACCESS DATA

Json Xml

Similar Datasets

UV-RIP experiments of ZNFX1 in VSV infected A549 cells

Project description:UV-RIP experiments of ZNFX1 in VSV infected A549 cells

| PRJNA549612 | ENA

L-azidohomoalanine peptide labelling for detection of newly synthesized peptides in A549 cells

Project description:AHA labelled peptides in control and UV treated cells in presence and absence of DAC51, SBI56, METTL3 siRNA.

2023-03-16 | MSV000091501 | MassIVE

HBX-HepG2 versus Control-HepG2 (no UV)

Project description:Mature microRNA expression profiling of HBx-expressing HepG2 cells versus Control cells. Free of UV sensitization. The mature microRNA expression profile of HBx-expressing HepG2 cells versus control cells in three independent biological repeated experiments (no UV). This study focuses only on human sequences. The complete data matrix is linked below as a supplementary file.

2010-05-24 | E-GEOD-17474 | biostudies-arrayexpress

UV-RIP experiments of ZNFX1 in VSV infected A549 cells

Project description:UV-RIP-seq was used to identify VSV-RNA associated with endogenous ZNFX1 in VSV infected A459 cells.

2019-08-30 | GSE132977 | GEO

UV timecourse

Project description:Transcriptome analysis of ultraviolet-radiation time course with paired treatment and control measurements provided groups of coordinately regulated genes and Gene Ontology processes. A set of genes significantly affected by UV was selected by linear modeling plus order-restricted inference profile matches; this gene list was used to find upstream sequence motifs that predict UV regulation of gene expression. Keywords: time course dose response stress response

2007-03-21 | GSE4509 | GEO

NIH 3T3 AHA p-c, SILAC p-c, inhibitor, and enrichment control experiments

Project description:We developed a pulse-chase method in where fully SILAC (heavy, medium-heavy and Light) labelled cells were pulsed with Azidohomoalanine (AHA) and then chased for different length of times. These experiments were performed with 0, 1, 2, 4, 8, 16 and 13 h of chase. Data from 3 biological replicates are provided. Each replicate contains data from 3 experiments (0, 1, 2 and 0, 4, 8 and 0, 16, 32 hours chase) in where the 0 h time point is always heavy labelled followed by the medium and light labelled time points. In addition, AHA p-c experiments were performed using only 0, 4 and 8 h chase times in combination with different inhibitor combinations (MG132, Actinomycin D and Wortmanninin + Bafilomycin A1) or control (DMSO). Also, a control enrichment data set was created. Here heavy SILAC labelled cells were pulsed with AHA before being mixed with light cells that were not pulsed with AHA. This was followed by the click reaction and enrichment of AHA containing proteins. Label-swap experiments were also performed. Finally, a SILAC p-c data set is provided. Here light cells were pulsed with heavy (Arg10, Lys8) amino acids and then split in two. One half of the cells was chased in medium (Arg6, Lys4) amino acids and the other part was directly frozen. Label-swap experiments and experiments using different pulse and chase times were also performed. All provided datasets are from mouse fibroblast cells (NIH 3T3).

2016-10-06 | PXD004929 | Pride

AdHBx UV vs AdEasy UV vs HepG2

Project description:Transcriptional profiling of HepG2 cells treated with UVC (254 nm) for 30 seconds and transduced with Adenoviral vector carrying the Hepatitis B Virus HBx gene vs Adenoviral vector control under UV treatment Keywords: Over-expression

2008-07-01 | GSE11064 | GEO

A549 cells and MSR-A549 cells

Project description:A549 cells and MSR-A549 cells

| PRJNA379619 | ENA

UV-B regulation of microRNAs in maize

Project description:Differential expression of microRNAs was studied in maize leaves after an 8-h-exposure under UV-B light. As a control, plants were kept in the greenhouse in the absence of UV-B

2013-10-31 | GSE19768 | GEO

Time course response of Synechocystis PCC 6803 to UV irradiation

Project description:The responses of the transcriptome of Synechocystis PCC 6803 to UV-irradiation were measured at time points over 36 h. Irradiation was provided by Sylvania soft white DuluzR compact fluorescent 23W bulbs (Osram Sylvania Ltd, Mississauga, Canada), a 20W RS UV-B medical light with a spectral maximum at 310 nm (model ‘TL’, Philips, Holland), and 15W black lights each with a spectral maximum at 368 nm (model F15T8-BL, General Electric, USA). Total quantum scalar irradiance was measured with a model QSL-100 meter (Biospherical Instruments Inc., San Diego, CA). The flux densities of the UV-A and UV-B components of the spectrum were measured with DIX series UV-B and 365A sensors, respectively, with a Spectroline DRC-100X digital radiometer (Spectronics Corporation, Westbury, NY). In these experiments full illumination represented a continuous photon flux density in the visible range of 330 μmol photons m-2 s-1, with UV-A and UV-B maxima of 3.8 x 10^6 and 0.8 x 10^6 mW m-2, respectively. All values reported were the incident fluxes within culture vessels at the immediate surface of the cell suspensions. Aliquot cultures (in duplicate) were harvested after 0, 15 min, 1 h, 3 h, 6 h, 12 h, 24 h and 36 h of UV-irradiation. For each time point, total RNA were isolated from stressed and unstressed cells, reverse-transcribed, differentially labelled (dye swapped), hybridized together (stressed versus unstressed samples) and analyzed with DNA glass microarrays (two slides per each time point) (Custom-commercial array : CyanoCHIP version 2.0, TAKARA). To identify differentially expressed genes, the median of the normalized ratio of Cy5/Cy3 intensity was calculated for each spot of the replicated dye-swap. The results of the analysis were carefully examined to exclude the dye effect between the 2 Cy-swapped arrays. Keywords: UV-irradiation, desiccation, Synechocystis PCC 6803, cyanobacteria, time course, transcription

2006-09-04 | GSE4606 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data