Phaeodactylum tricornutum proteome from SEC and IEX fractionation of native extract

ABSTRACT: Phaeodactylum tricornutum (UTEX 646) grown without silica were used to generate a native protein extract. Extraction involved multiple freeze/thaw cycles, sonication, and solubilization in buffer containing 1% NP40. Following chromatographic separation by SEC or IEX the fractions were reduced/alkylated and digested with trypsin for LC-MS/MS on an orbitrap fusion. Data were collected using a 75 minute top speed DDA method with CID.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Phaeodactylum Tricornutum (ncbitaxon:2850)

SUBMITTER: Edward Marcotte  

PROVIDER: MSV000091621 | MassIVE | Mon Apr 03 13:45:00 BST 2023

SECONDARY ACCESSION(S): PXD050670

REPOSITORIES: MassIVE