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Tetrahymena thermophila ciliary proteome fractionated by SEC and IEX

ABSTRACT: Cilia were isolated from Tetrahymena thermophila SB715 by pH shock. Soluble ciliary proteins were extracted in buffer containing 1% NP40 and fractionated by HPLC SEC or mixed bed IEX. All fractions were reduced/alkylated and digested with trypsin for LC-MS/MS on an Orbitrap Fusion using a DDA 75 minute top speed CID method. Data were processed using MS Blender.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Tetrahymena Thermophila (ncbitaxon:5911)

SUBMITTER: Edward Marcotte

PROVIDER: MSV000091648 | MassIVE | Wed Apr 05 09:35:00 BST 2023

SECONDARY ACCESSION(S): PXD050671

REPOSITORIES: MassIVE

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