Dataset Information

DSSO Crosslinking of IEX column fractions from Tetrahymena cilia extract fractionation.

ABSTRACT: Procedure details are reported in McCafferty et al. eLife2022;11e81977. Briefly cilia extract from Tetrahymena thermophila SB715 was fractionated on a mixed bed IEX HPLC column and all resulting fractions were crosslinked by addition of DSSO to 0.5mM. Following reduction, alkylation, trypsin digestion, and desalting, mass spectra were collected on an Orbitrap Fusion Lumos using a DDA MS2-MS3 method and spectra were processed in ProteomeDiscoverer 2.3 using the Xlinkx node to identify crosslinks. The look-up database for crosslink identification was generated from the same .RAW files using a standard Basic workflow to identify proteins present in the fractions.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Tetrahymena Thermophila (ncbitaxon:5911)

SUBMITTER: Edward Marcotte

PROVIDER: MSV000091761 | MassIVE | Thu Apr 20 15:31:00 BST 2023

SECONDARY ACCESSION(S): PXD041698

REPOSITORIES: MassIVE

ACCESS DATA

Similar Datasets

Project description:Over the past few decades cross-linking mass spectrometry (XLMS) has become a powerful tool for identification of protein-protein interactions and for gaining insight into the structures of proteins in living cells, tissues, and organelles. The development of new crosslinkers, enrichment strategies and data acquisition methods led to the establishment of numerous new software tools specifically for the analysis and interpretation of cross-linking data. We previously published one of these tools called MS Annika, a cross-linking search engine which can accurately identify cross-linked peptides in MS2 spectra from a variety of different MS-cleavable crosslinkers. In this publication we present an updated MS Annika and a new search algorithm that additionally supports processing of data from MS2-MS3-based approaches and identification of peptides from MS3 spectra. In the new MS2-MS3 search algorithm, MS3 spectra are matched to their corresponding precursor doublet peak in the MS2 scan to identify the crosslink modification and the monoisotopic peptide mass. This information is then used to adjust the MS3 spectra for search with MS Amanda, our in-house developed peptide search engine, to identify the cross-linked peptides. Peptides that are identified in the MS2 scan and one or more of the associated product MS3 scans are re-scored with a novel scoring function to reflect the increased confidence. Finally, the detected cross-links are validated by estimating the false discovery rate (FDR) using a target-decoy approach. We evaluated the MS3-search-capabilities of MS Annika on five different datasets covering a variety of experimental approaches and compared it to XlinkX and MaXLinker, two other cross-linking search engines that support MS3 crosslink identification. Three of the datasets were benchmark datasets of synthetic peptides that allow calculation of an experimentally validated FDR, and we show that MS Annika detects up to 4 times more true unique crosslinks than MaXLinker and up to 35% more than XlinkX while simultaneously yielding less false positive hits and therefore a more accurate FDR than the other two search engines. Additionally, for the other two datasets we could show that MS Annika finds between 74% to 2.5 times more crosslinks at 1% estimated FDR and reveals protein-protein interactions that are not detected by either XlinkX or MaXLinker.

Dataset Information

DSSO Crosslinking of IEX column fractions from Tetrahymena cilia extract fractionation.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure