Metabolomic characterization of behaviorally manipulated ants with multiomic data integration

ABSTRACT: For manipulated ant collection, the sample was then placed in a pre-chilled, sterile 2 mL microcentrifuge tube kept in liquid nitrogen until long-term storage at -80 C. We collected sham ant samples in a similar manner, lifting them from the experiment box with forceps. We also collected three blank samples at the beginning, middle, and end of manipulated ant sampling, i.e., after the first, 10th, and 20th manipulated ants were found (n = 3). We made blanks by briefly touching the forceps to the floor of the sham treatment housing boxes, submerging them in liquid nitrogen, and swirling them in a microcentrifuge tube. The WCMC processed samples in three LC-MS/MS protocols broadly tailored toward different chemistries: biogenic amines, polyphenols and flavonoids, and complex lipids. From the 20 samples collected per infection and sham treatments, the WCMC collected polyphenol data for ten individual ant heads. They used the remaining ten heads per treatment for biphasic extractions, with each individual head used for both biogenic amines and lipids. Blank-collection samples were shared across the three LC-MS/MS chemistries. The WCMC produced quality-control (QC) pool samples consisting of all samples used per protocol and additional blank-extraction controls (and therefore distinct from our experimental blanks made during sample collection). These WCMC control samples were run at the beginning and every ten samples thereafter per protocol. All LC-MS/MS protocols collected MS/MS data in a top-four data-dependent-acquisition (DDA) mode in both electrospray ionization (ESI) positive and negative modes. See associated publication for full details.

INSTRUMENT(S): 6546 Q-TOF LC/MS (Agilent instrument model), 6530A Q-TOF LC/MS, Q Exactive

ORGANISM(S): Camponotus Floridanus (ncbitaxon:104421) Ophiocordyceps Camponoti-floridani (ncbitaxon:2030778)

SUBMITTER: Charissa de Bekker   Ian Will  

PROVIDER: MSV000091768 | MassIVE | Fri Apr 21 07:30:00 BST 2023

REPOSITORIES: MassIVE