Dataset Information

An Orphan-Moonlighting Gene PRL-1 Enhances Photosynthetic Efficiency and Productivity in Plants

ABSTRACT: Increasing photosynthetic efficiency remains a major goal across the globe given its central role in CO2 assimilation. Here, we identified a nuclear genome-residing orphan gene, comprised of 3 exons, two with apparent endophytic origins and the third being a highly conserved fragment of the RIBULOSE BISPHOSPHATE CARBOXYLASE/ OXYGENASE LARGE SUBUNIT (RuBisCo). This novel gene, here-on referred to as Populus RuBisCo-like (PRL-1) gene localizes in chloroplast, endoplasmic reticulum and nuclear compartments of plant cells, acts as a transcriptional repressor and modulates adaptation to fluctuating light. P. trichocarpa genotypes with high PRL-1 expression levels quickly dissipated non-photochemical quenching (NPQ) upon transitioning from high to low light and rapidly induced NPQ upon returning to high light. The dynamic modulation of NPQ yields high quantum efficiency of linear electron transport (PSII) and 10-20% increased quantum efficiency of CO2 assimilation (CO2). The enhanced photosynthesis efficiency corresponded with increased plant height and biomass in P. trichocarpa with up to 35 % and 100% increases under field and greenhouse conditions. Transgenic Arabidopsis plants heterologously expressing the PRL-1 gene gained up to 200% in biomass and 97% in seed yield under greenhouse conditions. Taken together, PRL-1 presents a novel and trackable target for increasing photosynthetic efficiency in plants.

INSTRUMENT(S): Q Exactive Plus

ORGANISM(S): Populus Trichocarpa (ncbitaxon:3694)

SUBMITTER: Paul Abraham

PROVIDER: MSV000091785 | MassIVE | Mon Apr 24 05:32:00 BST 2023

SECONDARY ACCESSION(S): PXD041771

REPOSITORIES: MassIVE

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An orphan gene BOOSTER enhances photosynthetic efficiency and plant productivity.

Feyissa Biruk A BA de Becker Elsa M EM Salesse-Smith Coralie E CE Shu Mengjun M Zhang Jin J Yates Timothy B TB Xie Meng M De Kuntal K Gotarkar Dhananjay D Chen Margot S S MSS Jawdy Sara S SS Carper Dana L DL Barry Kerrie K Schmutz Jeremy J Weston David J DJ Abraham Paul E PE Tsai Chung-Jui CJ Morrell-Falvey Jennifer L JL Taylor Gail G Chen Jin-Gui JG Tuskan Gerald A GA Long Stephen P SP Burgess Steven J SJ Muchero Wellington W

Developmental cell 20241203 5

Organelle-to-nucleus DNA transfer is an ongoing process playing an important role in the evolution of eukaryotic life. Here, genome-wide association studies (GWAS) of non-photochemical quenching parameters in 743 Populus trichocarpa accessions identified a nuclear-encoded genomic region associated with variation in photosynthesis under fluctuating light. The identified gene, BOOSTER (BSTR), comprises three exons, two with apparent endophytic origin and the third containing a large fragment of pl ...[more]

PMID: 39631390

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