Proteomics

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The human cerebrospinal fluid lipoproteome


ABSTRACT: Lipoproteins in human cerebrospinal fluid (CSF-Lps) were exchange labeled with a rhodamine-containing phospholipid and size distribution determined using a fluorescent lipoprotein profiler. CSF-Lps within each fraction were isolated using lipid removal agent. Lipid-bound proteins were digested directly off the resin, collected, and analyzed using bottom-up mass spectrometry (LC-MS/MS). Computational analysis of protein abundance and co-migration patterns were used to generate protein network maps and identify potential CSF-Lp subspecies.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: John T. Melchior  

PROVIDER: MSV000091813 | MassIVE | Thu Apr 27 10:18:00 BST 2023

REPOSITORIES: MassIVE

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