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Syncytin1 sequesters viral LF2 to enhance the Epstein-Barr virus lytic phase

ABSTRACT: Previously, we identified Syncytin1 to be expressed in human B cells and enhance EBV ability to undergo lytic replication. To gain mechanistic insight, we performed liquid chromatography tandem mass spectrometry (LC-MS/MS) on tryptic peptides from immunoprecipitated FLAG-tagged Syncytin-1 in latently infected EBV+ Burkitt lymphoma cells. This revealed a number of cellular proteins as potential Syncytin-1 interacting partners during latency and/or during the lytic phase as well as two EBV lytic phase proteins, the viral protein kinase and LF2, a negative regulator of EBV lytic gene transcription. We now find that Syncytin1 reduces the association between LF2 and the EBV replication and transcription activator to promote EBV lytic gene expression.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Human Herpesvirus 4 (strain B95-8) (ncbitaxon:10377) Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Michael T. McIntosh, Ph.D.

PROVIDER: MSV000091949 | MassIVE | Mon May 15 12:30:00 BST 2023

REPOSITORIES: MassIVE

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