Dataset Information

A salivary GMC oxidoreductase of Manduca sexta re-arranges the green leaf volatile profile of its host plant

ABSTRACT: Green leaf volatiles (GLVs) are short-chain oxylipins that are emitted from plants in response to stress. Previous studies have shown that oral secretions (OS) of the tobacco hornworm Manduca sexta, introduced into plant wounds during feeding, catalyze the re-arrangement of GLVs from Z-3- to E-2-isomers. This change in the volatile signal however is bittersweet for the insect as it can be used by their natural enemies, as prey location cue. Here we identified a (3Z):(2E)-hexenal isomerase (Hi-1) in M. sexta OS that catalyzes the conversion of the GLV Z-3-hexenal to E-2-hexenal. Hi-1 mutants that were raised on a GLV27 free diet showed developmental disorders, indicating that Hi-1 also metabolizes other substrates important for the insects development. Phylogenetic analysis placed Hi-1 within the GMCb-subfamily and showed that Hi-1 homologs from other lepidopterans could catalyze similar reactions. Our results indicate that Hi- 1 not only modulates the plants GLV-bouquet but also functions in insect development.

INSTRUMENT(S): Q-TOF (Micromass instrument model)

ORGANISM(S): Manduca Sexta (ncbitaxon:7130)

SUBMITTER: Gertjan Kramer

PROVIDER: MSV000092011 | MassIVE | Tue May 23 03:12:00 BST 2023

REPOSITORIES: MassIVE

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Publications

A salivary GMC oxidoreductase of Manduca sexta re-arranges the green leaf volatile profile of its host plant.

Lin Yu-Hsien YH Silven Juliette J M JJM Wybouw Nicky N Fandino Richard A RA Dekker Henk L HL Vogel Heiko H Wu Yueh-Lung YL de Koster Chris C Große-Wilde Ewald E Haring Michel A MA Schuurink Robert C RC Allmann Silke S

Nature communications 20230628 1

Green leaf volatiles (GLVs) are short-chain oxylipins that are emitted from plants in response to stress. Previous studies have shown that oral secretions (OS) of the tobacco hornworm Manduca sexta, introduced into plant wounds during feeding, catalyze the re-arrangement of GLVs from Z-3- to E-2-isomers. This change in the volatile signal however is bittersweet for the insect as it can be used by their natural enemies, as a prey location cue. Here we show that (3Z):(2E)-hexenal isomerase (Hi-1) ...[more]

PMID: 37380635

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Project description:Background: The best studied insect-symbiont system is that of aphids and their primary bacterial endosymbiont Buchnera aphidicola. Buchnera inhabits specialized host cells called bacteriocytes, provides nutrients to the aphid and has co-speciated with its aphid hosts for the past 150 million years. We have used a single microarray to examine gene expression in the pea aphid, Acyrthosiphon pisum, and its resident Buchnera. Very little is known of gene expression in aphids, few studies have examined gene expression in Buchnera, and no study has examined simultaneously the expression profiles of a host and its symbiont. Expression profiling of aphids, in studies such as this, will be critical for assigning newly discovered A. pisum genes to functional roles. In particular, because aphids possess many genes that are absent from Drosophila and other holometabolous insect taxa, aphid genome annotation efforts cannot rely entirely on homology to the best-studied insect systems. Development of this dual-genome array represents a first attempt to characterize gene expression in this emerging model system. Results: We chose to examine heat shock response because it has been well characterized both in Buchnera and in other insect species. Our results from the Buchnera of A. pisum show responses for the same gene set as an earlier study of heat shock response in Buchnera for the host aphid Schizaphis graminum. Additionally, analyses of aphid transcripts showed the expected response for homologs of known heat shock genes as well as responses for several genes with unknown functional roles. Conclusions: We examined gene expression under heat shock of an insect and its bacterial symbiont in a single assay using a dual-genome microarray. Further, our results indicate that microarrays are a useful tool for inferring functional roles of genes in A. pisum and other insects and suggest that the pea aphid genome may contain many gene paralogs that are differentially regulated. Keywords: Stress response

Dataset Information

A salivary GMC oxidoreductase of Manduca sexta re-arranges the green leaf volatile profile of its host plant

Publications

A salivary GMC oxidoreductase of Manduca sexta re-arranges the green leaf volatile profile of its host plant.

Similar Datasets

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