Acetylome, carbamylome, and phosphoproteome of RAW 264.7 cells treated with bacterial lipopolysaccharide
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ABSTRACT: Proteome, acetylome/carbamylome, and phosphoproteome of RAW 264.7 cells treated for 24 h with bacterial lipopolysaccharide. Samples were digested with trypsin in a buffer containing urea or sodium deoxycholate, labeled with tandem mass tags, and fractionated by high pH reverse phase chromatography. Samples were sequentially enriched by IMAC and anti-acetyllysine immunoaffinity capturing before analyzing by LC-MS/MS.
INSTRUMENT(S): Orbitrap Fusion Lumos
ORGANISM(S): Mus Musculus (ncbitaxon:10090)
SUBMITTER:
Ernesto S. Nakayasu
PROVIDER: MSV000092020 | MassIVE | Tue May 23 20:50:00 BST 2023
REPOSITORIES: MassIVE
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