Proteomics

Dataset Information

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ToxoNet: A high confidence map of protein-protein interactions in Toxoplasma gondii


ABSTRACT: The purpose of this project is to predict Toxoplasma gondii complexes from cofractionation datasets. In short, T. gondii lysates were fractionated utilizing reagent beads with different biochemical surface properties. 5 beads were utilized with each generating 60 fractions, resulting in a total of 300 fractions. T. gondii lysates were also subjected to fractionation by ion exchange chromatography (IEX), resulting in an additional 120 fractions. These fractions are then analyzed by liquid chromatography-mass spectrometry (LC-MS). Protein fractionation profiles are then utilized to predict protein complexes. See the sample processing protocol for experimental details.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Toxoplasma Gondii Me49

SUBMITTER: John Parkinson  

PROVIDER: MSV000092051 | MassIVE | Tue May 30 08:34:00 BST 2023

REPOSITORIES: MassIVE

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