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Mus musculus brown adipose tissue histone proteoforms

ABSTRACT: Histones were isolated from brown adipose tissue and liver from mice housed at 28, 22, or 8 C. Quantitative top- or middle-down approaches were used to quantitate histone H4 and H3.2 proteoforms. See published article for complimentary RNA-seq and RRBS datasets.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Mus Musculus (ncbitaxon:10090)

SUBMITTER: Nicolas L. Young

PROVIDER: MSV000092105 | MassIVE | Mon Jun 05 12:06:00 BST 2023

SECONDARY ACCESSION(S): PXD042736

REPOSITORIES: MassIVE

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Histone proteoform analysis reveals epigenetic changes in adult mouse brown adipose tissue in response to cold stress.

Taylor Bethany C BC Steinthal Loic H LH Dias Michelle M Yalamanchili Hari Krishna HK Ochsner Scott A SA Zapata Gladys E GE Mehta Nitesh R NR McKenna Neil J NJ Young Nicolas L NL Nuotio-Antar Alli M AM

Epigenetics & chromatin 20240427 1

<h4>Background</h4>Regulation of the thermogenic response by brown adipose tissue (BAT) is an important component of energy homeostasis with implications for the treatment of obesity and diabetes. Our preliminary analyses of RNA-Seq data uncovered many nodes representing epigenetic modifiers that are altered in BAT in response to chronic thermogenic activation. Thus, we hypothesized that chronic thermogenic activation broadly alters epigenetic modifications of DNA and histones in BAT.<h4>Results ...[more]

PMID: 38678237

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