Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Plasmodium yoelii zygotes and female gametocytes

ABSTRACT: Plasmodium yoelii zygotes or female gametocytes were purified by FACS and analyzed by DIA LC-MS.

INSTRUMENT(S): Orbitrap Eclipse

ORGANISM(S): Plasmodium Yoelii (ncbitaxon:5861)

SUBMITTER: Kristian Swearingen

PROVIDER: MSV000092154 | MassIVE | Tue Jun 13 13:07:00 BST 2023

SECONDARY ACCESSION(S): PXD042955

REPOSITORIES: MassIVE

ACCESS DATA

Json Xml

Similar Datasets

Comparing transcrional changes in Plasmodium yoelii infected to uninfected primary hepatocytes

Project description:GFP expressing Plasmodium yoelii (Py-GFP) infected, or naïve cultured primary hepatocytes were FACS sorted and re-suspended in TRIzol and RNA purified with RNAeasy kit (Qiagen).RNA was assessed for purity and quality using an Agilent 2100 Bioanalyzer and transcriptome analysis performed on Affymetrix GeneChip Mouse Transcriptome array 1.0. Data output was analyzed and visualized using Affymetrix Expression Console 4.0.

2019-05-10 | GSE130961 | GEO

Comparing transcrional changes in Plasmodium yoelii infected to uninfected primary hepatocytes

2021-10-18 | GSE186023 | GEO

Expression analysis of Plasmodium falciparum male and female gametocytes

Project description:Investigation of whole genome gene expression level in Plasmodium falciparum male and female mature gametocytes, and detection of any transcriptional differences between male and female gametocytes. The Plasmodium falciparum parasite with green fluorescent protein (GFP) expression under the control of alpha tubulin II promoter facilitated the separation of male and female gametocyte. This engineered parasite strain in this study are further described in Miao J, Fan Q, Parker D, Li X, Li J, et al. (2013) Puf Mediates Translation Repression of Transmission-Blocking Vaccine Candidates in Malaria Parasites. PLoS Pathog 9(4): e1003268. doi: 10.1371/journal.ppat.1003268

2016-01-28 | GSE77316 | GEO

Plasmodium berghei transcriptome for female gametocytes, male gametocytes, and asexual erythrocytic stages

Project description:Plasmodium berghei transcriptome for female gametocytes, male gametocytes, and asexual erythrocytic stages

2017-08-21 | GSE102167 | GEO

Next generation sequencing analysis of transcriptomes of Plasmodium falciparum male and female gametocytes

Project description:Investigation of overall expression level in Plasmodium falciparum male and female mature gametocytes, and detection of any transcriptional differences between male and female gametocytes. The Plasmodium falciparum parasite with green fluorescent protein (GFP) expression under the control of alpha tubulin II promoter facilitated the separation of male and female gametocyte. This engineered parasite strain in this study are further described in Miao J, Fan Q, Parker D, Li X, Li J, et al. (2013) Puf Mediates Translation Repression of Transmission-Blocking Vaccine Candidates in Malaria Parasites. PLoS Pathog 9(4): e1003268. doi: 10.1371/journal.ppat.1003268

2016-01-30 | GSE77398 | GEO

Transcriptome analysis of immature and mature male and female gametocytes of the malaria parasite Plasmodium falciparum

Project description:In the human host, Plasmodium falciparum parasites propagate asexually in erythrocytes causing the symptoms of malaria. However, in every asexual cycle, a small proportion of the parasites commits to sexual differentiation, which is critical for transmission. During a period of about 10 days, the sexually committed parasites either develop into male or female gametocytes. In this study, gametocytes carrying an endogenously GFP tagged version of the female specifically expressed ABCG2 gene were FACS sorted to separate male (GFP low) and female (GFP high) gametocyte populations at different days of development. RNA was subsequently extracted from these populations as well as from asexual parasites as controls and subjected to directional RNAseq. These data reveal the transcriptional differences between asexual parasite stages and developing gametocytes, but also between male and female gametocytes as well as for each sex during the course of differentiation. In particular, male gametocytes undergo distinct transcriptional transitions when comparing the transcriptome between day 4, 6 and 10 of gametocytogenesis. In contrast, female gametocyte transcriptomes are more static during the course of gametocytogenesis and only show minimal variation. This is the first study to longitudinally monitor gene expression in developing male and female gametocytes

2021-07-31 | GSE180985 | GEO

Comparative proteomic and RNA-seq analyses identify glypican-3 as a hepatocyte host cell receptor for Plasmodium falciparum sporozoite invasion

Project description:Understanding the liver stage invasion and development of Plasmodium falciparum parasites is difficult due to limitations in current methodologies, namely, a hepatocyte cell line that supports the invasion of sporozoites. In this work we seek to develop and characterize a subclone of the HC-04 cell line that supports higher invasion rates than the parental line. We make use of both RNA-seq and LC-MS/MS proteomics to characterize cells grown in two different mediums. Finally, we identify and validate a surface protein, glypican-3, which is deferentially expressed between parental and subclone cell lines, when compared to HepG2 cells, and demonstrate that antibodies against glypican-3 decrease parasite invasion.

2019-03-02 | E-MTAB-6919 | biostudies-arrayexpress

PFG controls gene expression in female gametocytes cooperatively with AP2-FG [PFG_ChIP-seq]

Project description:AP2-FG is a female-specific transcription factor (TF) that plays an essential role in female gametocyte development. AP2-FG activates hundreds of genes by binding to a female-specific ten-base cis-acting element. Here, we report that in the rodent malaria parasite Plasmodium berghei, another female-specific TF designated as a partner of AP2-FG (PFG), controls gene expression in female gametocytes cooperatively with AP2-FG. Transcriptional mechanisms were analyzed in Plasmodium berghei female gametocytes.

2024-01-22 | GSE226021 | GEO

PFG controls gene expression in female gametocytes cooperatively with AP2-FG [AP2-FG_DIP]

2024-01-22 | GSE226020 | GEO

The proteomes of plasmodium falciparum mature male and female gametocytes

Project description:Gametocytes are obligate for the transmission of malaria parasite to mosquito. This study reports the first comprehensive proteomes of accurately separated mature male and female Plasmodium falciparum gametocytes using a transgenic line. the proteomes revealed significant differences that are consistent with the divergent functions of the two sexes. While the male proteome is enriched in proteins associated with flagella and genome replication, the female proteome is more abundant in proteins involved in metabolism, translation and organellar functions.

2017-01-30 | PXD003556 | Pride

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data