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Subcytoplasmic location of translation controls protein output

ABSTRACT: Supporting raw data of HEK293T cells proteomics analysis. (related to Horste et al 2023 - DOI: 10.1016/j.molcel.2023.11.025). TMTpro channel used for analysis 132c and 133n.

INSTRUMENT(S): Orbitrap Eclipse

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Christine Mayr

PROVIDER: MSV000092176 | MassIVE | Thu Jun 15 09:46:00 BST 2023

REPOSITORIES: MassIVE

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Subcytoplasmic location of translation controls protein output.

Horste Ellen L EL Fansler Mervin M MM Cai Ting T Chen Xiuzhen X Mitschka Sibylle S Zhen Gang G Lee Flora C Y FCY Ule Jernej J Mayr Christine C

Molecular cell 20231201 24

The cytoplasm is highly compartmentalized, but the extent and consequences of subcytoplasmic mRNA localization in non-polarized cells are largely unknown. We determined mRNA enrichment in TIS granules (TGs) and the rough endoplasmic reticulum (ER) through particle sorting and isolated cytosolic mRNAs by digitonin extraction. When focusing on genes that encode non-membrane proteins, we observed that 52% have transcripts enriched in specific compartments. Compartment enrichment correlates with a c ...[more]

PMID: 38134885

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