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WNT2B is required for development and function of human intestine

ABSTRACT: The experiment goal was to identify differentially expressed proteins associated with WNT2B mutation p.R69* in human intestinal epithelia. Skin fibroblasts from a patient with the mutation were used to establish human intestinal organoids (HIOs). The hESC H1 cell line was used to generate wild-type WNT2B HIOs for comparison. The HIOs were transplanted into mice to promote maturation for 8 weeks, followed by dissection and crypt isolation to seed ex vivo epithelial (enteroid) cultures. The enteroid cultures were harvested for analysis by tandem mass tag spectrometry.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Jennifer Foulke-Abel

PROVIDER: MSV000092189 | MassIVE | Fri Jun 16 06:22:00 BST 2023

SECONDARY ACCESSION(S): PXD043055

REPOSITORIES: MassIVE

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