Dataset Information

A general multipronged activation approach for natural product discovery in Actinomycetes 54 actinobacterial strains with genetic and cultivation based activation

ABSTRACT: LC-MS/MS fermentation extract collection of 54 diverse and non-domesticated actinobacterial strains interrogated with a one-step integrase-mediated genetic- and cultivation-based approach to perturb and activate metabolite production in actinobacteria that has significantly increased accessible metabolite space of the collection by nearly two-fold and selected metabolite yields by up to >200-fold. Total of 124 unique strain-activator combinations and 459 mutants fermented in 3-5 media.

INSTRUMENT(S): 6540 Q-TOF LC/MS

ORGANISM(S): Streptomyces Viridochromogenes (ncbitaxon:1938) Streptomyces Abikoensis (ncbitaxon:97398) Thermoactinomyces Vulgaris (ncbitaxon:2026) Streptomyces Albogriseolus (ncbitaxon:1887) Streptomyces Sampsonii (ncbitaxon:42239) Streptomyces Ardesiacus Streptomyces Fradiae (ncbitaxon:1906) Streptomyces Filipinensis (ncbitaxon:66887) Streptomyces Niveiscabiei (ncbitaxon:164115) Streptomyces Odonnellii (ncbitaxon:1417980) Streptomyces Anandii (ncbitaxon:285454) Streptomyces Thermocarboxydus (ncbitaxon:59299) Streptomyces Flaveolus (ncbitaxon:67297) Streptomyces Albofaciens (ncbitaxon:66866) Micromonospora Maris Streptomyces Luridiscabiei (ncbitaxon:164114) Streptomyces Lavendulae (ncbitaxon:1914) Streptomyces (ncbitaxon:1883) Spirillospora Tritici Streptomyces Kronopolitis (ncbitaxon:1612435) Streptomyces Niveoruber (ncbitaxon:68243) Streptomyces Cyaneochromogenes Streptomyces Lunaelactis (ncbitaxon:1535768) Streptomyces Hirsutus (ncbitaxon:35620) Streptomyces Jeddahensis (ncbitaxon:1716141) Streptomyces Tendae Strain Atcc 19812 Micromonospora Oryzae (ncbitaxon:1530046) Streptomyces Nigrescens (ncbitaxon:1920) Streptomyces Durhamensis (ncbitaxon:68194) Streptomyces Variabilis (ncbitaxon:67372) Micromonospora Aurantiaca (ncbitaxon:47850) Streptomyces Tendae (ncbitaxon:1932) Micromonospora Maritima (ncbitaxon:986711) Streptomyces Globisporus (ncbitaxon:1908) Dactylosporangium Solaniradicis (ncbitaxon:1758122)

SUBMITTER: Fong Tian Wong

PROVIDER: MSV000092237 | MassIVE | Thu Jun 22 00:30:00 BST 2023

REPOSITORIES: MassIVE

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Exploring a general multi-pronged activation strategy for natural product discovery in Actinomycetes.

Tay Dillon W P DWP Tan Lee Ling LL Heng Elena E Zulkarnain Nadiah N Ching Kuan Chieh KC Wibowo Mario M Chin Elaine Jinfeng EJ Tan Zann Yi Qi ZYQ Leong Chung Yan CY Ng Veronica Wee Pin VWP Yang Lay Kien LK Seow Deborah C S DCS Lim Yi Wee YW Koh Winston W Koduru Lokanand L Kanagasundaram Yoganathan Y Ng Siew Bee SB Lim Yee Hwee YH Wong Fong Tian FT

Communications biology 20240106 1

Natural products possess significant therapeutic potential but remain underutilized despite advances in genomics and bioinformatics. While there are approaches to activate and upregulate natural product biosynthesis in both native and heterologous microbial strains, a comprehensive strategy to elicit production of natural products as well as a generalizable and efficient method to interrogate diverse native strains collection, remains lacking. Here, we explore a flexible and robust integrase-med ...[more]

PMID: 38184720

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