Project description:With this experiment we sought to characterise the effect of Extracellular Vesicle (EV) mediated transfer of mitochondria from Neural Stem Cells (NSCs) to macrophages. Briefly, macrophages were obtained from the bone marrow of C57BL/6 mice and stimulated with 50 ng/ml LPS. Macrophages were treated with EVs derived from Neural Stem Cells for 6 hours after which RNA was extracted for microarray analysis.
Project description:We performed single nucleus RNA-seq on rat fetal lungs from: control (normal), hypoplastic (injury), and hypoplastic+ amniotic fluid stem cell extracellular vesicles treated (injury+treatment).
Project description:Fetal hypoplastic lungs have multilineage inflammation that is reversed by amniotic fluid stem cell extracellular vesicle treatment
Project description:Extracellular Vesicle Transcriptome in Ischemic Stroke Rat with Intra-arterial Transplantation of Human Neural Stem Cells (microRNA)
Project description:Expression of 757 genes related to neuroinflammation was analyzed across 7 treatment groups of male mice to investigate the neuroprotective effects of two different human neural stem cell (hNSC)-derrived extracellular vesicles (EVs) against chemoradiotherapy for brain cancer; the two stem cell lines are Shef6 and UCI-191, kindly provided from our collaborators.