Proteomics Analysis of the UMK2 Mutation in Arabidopsis thaliana seeds

ABSTRACT: We compared the proteome of dry as well as germinating seeds of Arabidopsis thaliana wild type Col-0 with the respective proteomes from an umk2 (AT4G25280) frameshift mutant. Germinating seeds were incubated for 48 hours in 1/2-MS medium in Petri dishes at 4 degree Celsius followed by 24 hours of a phytochamber under long-day conditions. Protein extraction, digestion by SP3 workflow, and LC-IMS-MS/MS analysis as described in https://doi.org/10.1038/s41477-022-01308-6.

INSTRUMENT(S): timsTOF Pro

ORGANISM(S): Arabidopsis Thaliana (ncbitaxon:3702)

SUBMITTER: Hans-Peter Braun   Nils Rugen  

PROVIDER: MSV000092331 | MassIVE | Mon Jul 03 00:39:00 BST 2023

SECONDARY ACCESSION(S): PXD043459

REPOSITORIES: MassIVE