Project description:Long-term effects of imatinib, a chemotherapy agent, on S. cerevisiae cells were investigated. Yeast cells were grown in the absence and presence of 400 mg/l imatinib in fully controlled bioreactors, in triplicates. Control cultures were described before, E-MTAB-6634.
Project description:This study newly identified Tripelennamine (TA) as an inhibitor of yeast meiosis and sporulation. To examine if and how exposure of sporulating yeast cells to TA changes the meiotic transcriptional program cells were sporulated for 0, 4, and 8 hours in the presence or absence of 100 uM TA.
Project description:When eukaryotic cells are deprived of amino acids, uncharged tRNAs accumulate and activate the conserved GCN2 protein kinase. We examine how yeast growth and tRNA charging or aminoacylation is affected during amino acid depletion in the presence and absence of GCN2. tRNA charging is measured using a microarray technique which allows for simultaneous measurement of all cytosolic tRNAs. A fully prototrophic and its isogenic GCN2 deletion strain were used.
Project description:We applied extensive assays--repeated over several genetic backgrounds and environments--to reveal some general features of the loss of heterozygosity (LOH) in yeast. We found that LOH: was most frequent in homozygotes, was more densely distributed on shorter chromosomal arms, depended on the presence of some repetitive elements but not the intensity of transcription, occured also in absence of growth although at a low rate. Our findings uncover new features of LOH and help to interpret those already known.
Project description:Growth assay in the presence of Selenomethionine that uses the barcoded collections of yeast gene modification (deletion or DamP) to identify strains that are hypersensitive to the presence of the aminoacid.
Project description:Growth assay in the presence of a toxic chemical (sr7575) that uses the barcoded collections of yeast gene deletions (haploid, diploid, DamP) to identify deletion strains that are hypersensitive to the drug.