Project description:In this set of experiments we identified the main scaffolding component of centriolar satellites, PCM1 or Cmb in Drosophila as a proximity interactor of the centriolar structural protein Sas4 by BioID in S2 cells and subsequently explored the Cmb proximity interactome by direct TurboID in S2 cells and indirect, GFP nanobody-targeted, TurboID in Drosophila testes.
Project description:SH-SY5Y cells were transfected with either BioID-GFP-NLS control or BioID-Flag-ERRg expressing adenoviruses and isolated with Trizol after 48 hours to compare gene expression and find potential ERRg targets specific to neuron-like cells.
Project description:ChIP-chip experiment for nuclear pore proteins Nup153 and Mtor in Drosophila S2 and Kc cells. This experiment is related to E-MEXP-2523.
Project description:In order to identify interaction partner of the Drosophila melanogaster TFIIA protein, we have immunoprecipitated an endogenously 3xFLAG-AID tagged TFIIA-L from Drosophila Schneider S2 cells
Project description:We report the MNase-diestion coupled to Next Generation Sequencing of Wild type Drosophila S2 cells or S2 cells over-expressing polycomb protein PH