MassSpecPreppy - an end-to-end solution for automated protein concentration determination and flexible sample digestion for proteomics applications

ABSTRACT: In proteomics, fast, efficient and highly reproducible sample preparation are of utmost importance, particularly in view of fast scanning mass spectrometers enabling analyses of large sample series. To address this need, we have developed the web application MassSpecPreppy that operates on the open science OT-2 liquid handling robot from Opentrons. This platform can prepare up to 96 samples at once, performing tasks like BCA protein concentration determination, sample digestion with normalization, reduction/alkylation, enzymatic digestion, and peptide elution or loading specified peptide amounts onto Evotips in an automated and flexible manner.

INSTRUMENT(S): timsTOF Pro 2, Orbitrap Exploris 480

ORGANISM(S): Saccharomyces Cerevisiae Strain C13-aby.s86 Escherichia Coli K-12 (ncbitaxon:83333) Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Stephan Michalik  

PROVIDER: MSV000092489 | MassIVE | Thu Jul 20 04:19:00 BST 2023

REPOSITORIES: MassIVE