Proteomics

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Subtiligase N-terminomics for identification of cleavage sites in mice brain and immunoprecipitated PrP

ABSTRACT: Subtiligase-based N-terminomics (Abu N-terminal tagging) of whole mouse brain and immunoprecipitated prion protein. Mouse expressing either WT or mutant (S3) form of PrP were used in this study. Five replicates for single brain experiments and one replicate for combined brains approach are included. For labeling of PrP, 2 biological replicates are part of the study.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Mus Musculus (ncbitaxon:10090)

SUBMITTER: Olivier Julien  

PROVIDER: MSV000092542 | MassIVE | Thu Jul 27 12:41:00 BST 2023

REPOSITORIES: MassIVE

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Publications

Application of N-Terminal Labeling Methods Provide Novel Insights into Endoproteolysis of the Prion Protein <i>in Vivo</i>.

Gomez-Cardona Erik E   Eskandari-Sedighi Ghazaleh G   Fahlman Richard R   Westaway David D   Julien Olivier O  

ACS chemical neuroscience 20231214 1

Alternative α- and β-cleavage events in the cellular prion protein (PrP<sup>C</sup>) central region generate fragments with distinct biochemical features that affect prion disease pathogenesis, but the assignment of precise cleavage positions has proven challenging. Exploiting mouse transgenic models expressing wild-type (WT) PrP<sup>C</sup> and an octarepeat region mutant allele (S3) with increased β-fragmentation, cleavage sites were defined using LC-MS/MS in conjunction with N-terminal enzyma  ...[more]

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