Project description:Aim of the project was to identify interactors of the phytochemical papaverine, modified with a biotin moiety, from HEK293 total cell extract by LC-MS/MS.
Project description:The present study aims to synthesize zinc oxide nanoparticles using propolis extract and evaluate its effect on zebrafish immune response following caudal fin amputation model on zebrafish (Danio rerio). The phytochemical composition of extract was identified using Gas Chromatography Mass Spectrometry (GC-MS). The synthesized Pro-ZnO NPs were characterized using UV-Vis spectrophotometer, Fourier transform infrared (FTIR) and transmission electron microscopy (TEM) analysis. The toxicity of Pro-ZnO NPs was evaluated in zebrafish larvae, with the LC50 values of 4 mg/L which classified as toxic. Male zebrafish (n = 5 per group, triplicate) underwent caudal fin amputation and were divided into two groups to assess their immunological response: a control group and a Pro-ZnO 2 mg/L treatment group for a duration of four days. Total RNA was extracted from the caudal fin blastema four days post-amputation (dpa), and subsequently subjected to strand-specific mRNA sequencing using Illumina Platform. The principal component analysis (PCA) yielded a clear delineation between the ZnO-treated group and the control group, thereby indicating a distinct grouping pattern.
Project description:Passiflora mollissima commonly known as “banana passion fruit” is usually consumed as fresh food or processed products, being seeds and peel the main by-products of the industrial processing. The potentially bioactive metabolites from banana passion fruit PLE-extract seeds have been recently characterized by HPLC-HRMS after sequential pressurized liquid extraction (PLE) To apply a Foodomics approach to study the effects of a banana passion fruit seeds PLE-extract (with high antioxidant capacity and enriched in phenolic-type compounds) on the transcriptome and metabolome of HT-29 colon cancer cells.