Native mass spectrometry of an allosteric heterodimer of SARS-CoV-2

ABSTRACT: The capability of native MS to probe protein-protein and protein-ligand interactions was exemplified with the allosteric heterodimer from SARS-CoV-2 consisting of the nonstructural proteins (nsp) nsp10 and nsp16. Complex dynamics, allostery, and ligand binding were shown. A metadata Excel file, 'nsp1016_metadata.xlsx' is included to help orient users which raw files were used for which analyses. Supplementary binding affinity calculations for ligand binding results are found in an Excel file found in directory 'Kd_analysis'. UniDec (Universal Deconvolution of Mass and Ion Mobility Spectra) configuration '.dat' files are found in their respective directories.

INSTRUMENT(S): SYNAPT G2-Si, Orbitrap Exploris 480, Q Exactive HF

ORGANISM(S): Escherichia Coli Bl21(de3) (ncbitaxon:469008)

SUBMITTER: Mowei Zhou  

PROVIDER: MSV000092776 | MassIVE | Mon Aug 28 22:08:00 BST 2023

REPOSITORIES: MassIVE