Project description:The objective of the present study was to identify the nutrient utilization and the SCFA production potential of gut microbes during the first year of life. The 16S sequencing data represents 100 mother-child pairs, longitudinally for the infants (0, 3mo, 6mo and 12mo) and mothers 18 weeks pregnancy. We wanted to identify the SCFA composition in pregnant woman and their infants through the first year of life, and their correlation to gut bacteria and other influencal factors. Metaproteomics on selected infants were analyzed to look for nutrient sources used by potential SCFA producers.
Project description:<p>Infancy is a critical period for the colonization of the gut microbiome. However, xenobiotic effects on gut microbiome development in early life remain poorly understood. Here, we recruit 146 mother-infant pairs and collect stool samples at 3, 6, and 12 months after delivery for amplicon sequencing (N = 353), metagenomics (N = 65), and metabolomics (N = 198). Trace elements in maternal hair samples (N = 119) affect the alpha diversity of the infant gut microbiome. Shannon diversity in 3-month-old infants is correlated positively with selenium and negatively with copper, and the relative abundance of Bifidobacterium increases under high exposure to aluminum and manganese. During the first year of life, infants and their paired mothers have distinct microbial diversity and composition, and their bacterial community structures gradually approach. There are 56 differential metabolites between the first and second postpartum visits and 515 differential metabolites between the second and third visits. The typical profile of antibiotic resistance genes (ARGs) differs significantly between infants and their mothers. High copper and arsenic exposure may induce the enrichment of ARGs in the infant gut. Our findings highlight the dynamics of the gut microbiome, metabolites, and ARG profiles of mother-infant pairs after delivery, associated with prenatal exposure to trace elements.</p>
Project description:Blood collected from infants at week 6 and week 7 of life to profile how the transcriptome is changed following routine early life vaccination. Paired faecal metagenomic data is available for these infants under BioProject PRJNA807448 at the Sequence Read Archive.
Project description:Outbred D.melanogaster populations subjected to >300 generations of natural selection on either control, or 12% ethanol, or variable food (2 replicates each) and exposed, as first instar larvae, to either water control or 12% ethanol.