Characterization of molecular partners and physiological functions of the conserved RNA binding protein Rbp3

ABSTRACT: The RNA recognition motif (RRM) domain proteins are crucial RB (Rbps) in all domains of life. In cyanobacteria, proteins with a single RRM domain were suggested to be involved in mRNA targeting to the thylakoid membrane and the acclimation to certain stress conditions. The model cyanobacterium Synechocystis sp. PCC 6803 contains a small family of 3 genes encoding RRM-domain containing Rbps, Rbp1, Rbp2 and Rbp3. With a focus on Rbp3, here, we addressed the molecular details of its functions and the associated physiological consequences. To elucidate the set of interacting proteins, co-immunoprecipitation experiments were performed with a strain expressing an Rbp3 with a triple FLAG tag added to its C-terminus.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Synechocystis Sp. Pcc 6803 (ncbitaxon:1148)

SUBMITTER: Wolfgang R. Hess  

PROVIDER: MSV000092937 | MassIVE |

SECONDARY ACCESSION(S): PXD045584

REPOSITORIES: MassIVE