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NCLX proximity biotinylation screen

ABSTRACT: The mitochondrial sodium/calcium exchanger, NCLX, is critical to maintaining mitochondrial calcium homeostasis, yet little remains known about the mechanisms regulating its function. Here, we performed proximity biotinylation screening to identify the NCLX interactome and potential protein regulators of NCLX function by expressing a human NCLX-BioID2 fusion protein, or BioID2 alone, in AC16 cardiomyocytes and in HeLa cells. Biotinylated proteins were identified via affinity purification mass spectrometry.

INSTRUMENT(S): Q Exactive Plus

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: John Elrod

PROVIDER: MSV000093004 | MassIVE |

SECONDARY ACCESSION(S): PXD045834

REPOSITORIES: MassIVE

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TMEM65 regulates and is required for NCLX-dependent mitochondrial calcium efflux.

Garbincius Joanne F JF Salik Oniel O Cohen Henry M HM Choya-Foces Carmen C Mangold Adam S AS Makhoul Angelina D AD Schmidt Anna E AE Khalil Dima Y DY Doolittle Joshua J JJ Wilkinson Anya S AS Murray Emma K EK Lazaropoulos Michael P MP Hildebrand Alycia N AN Tomar Dhanendra D Elrod John W JW

Nature metabolism 20250408 4

The balance between mitochondrial calcium (mCa2+) uptake and efflux is essential for ATP production and cellular homeostasis. The mitochondrial sodium-calcium exchanger, NCLX, is a critical route of mCa2+ efflux in excitable tissues, such as the heart and brain, and animal models support NCLX as a promising therapeutic target to limit pathogenic mCa2+ overload. However, the mechanisms that regulate NCLX activity are largely unknow ...[more]

PMID: 40200126

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The role of stretch, tachycardia and sodium-calcium exchanger in induction of early cardiac remodeling

Project description:Accute stretch and tachycardia are capable of inducing pathological excitation transcription coupling - an early invent before structural cardiac remodeling which transitions to heart failure. The sodium calcium exchanger is a key player in maintaining calcium homeostasis and is implicated in pathological signaling during heart failure. Neonatal rat ventricular cardiomyocytes (NRVCM) were subjected to mechanical stress and high freqency elecrical stimulation to study genes regulated during the early phase of trigger induced cardiac remodeling. Inhibition of the sodium calcium exchanger was used in an attempt to supress the early remodeling process and gain insight into the pathological signaling pathway.

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Identification of TbUnc119-interacting proteins by proximity-based biotinylation

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Calcium-dependent proximal biotinylation via Cal-ID

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