Dataset Information

Integrated analysis of transcriptomics and proteomics approach the importance of ABA-dependent pathways in Mesembryanthemum Crystallinum leaf during the salt induced C 3 to CAM transition

ABSTRACT: Mesembryanthemum Crystallinum, a facultative CAM plant, shifts from C 3 to CAM photosynthesis under salt stress, enhancing water use efficiency due to inverse stomatal patterns. Exploring the mechanisms of this transition could improve salt tolerance in C 3 crops. We used transcriptomics, proteomics, and targeted metabolomics every 8 hours to track molecular shifts during this transition.

INSTRUMENT(S): Vanquish LC and Altis mass spectrometer (Thermo Scientific, Bremen, Germany)

ORGANISM(S): Mesembryanthemum Crystallinum

SUBMITTER: Sixue Chen

PROVIDER: MSV000093065 | MassIVE | Sat Oct 07 11:11:00 BST 2023

REPOSITORIES: MassIVE

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Project description:Backgroud: microRNA (miRNA) is implicated in plant development processes, playing pivotal roles in plant adaptation to environmental stresses. Salicornia europaea, a salt mash euhalophyte, is a good model plant to study salt adaptation mechanisms. It is also attractive in being vegetables, forage and oilseed that can be used for saline land reclamation and biofuel precursor production on marginal lands. However, none of the miRNAs from S. europaea have been identified so far. Results: Deep sequencing was performed to investigate small RNA transcriptome of S. europaea. Two hundred and twelve conserved miRNAs comprising 51 families and 31 novel miRNAs (including 7 miRNA star sequences) belonging to 30 families were identified. Interestingly, about half (13 out of 31) of the novel miRNAs were only detected in salt-treated samples. The expression of 43 conserved and 13 novel miRNAs changed significantly in response to salinity. In addition, 53 conserved miRNAs and 13 novel miRNAs were differentially expressed between shoots and roots. Furthermore, a total of 306 and 195 S. europaea unigenes were predicted to be targets of 41 conserved and 29 novel miRNA families, respectively. These targets encode a wide range of proteins, and genes involved in transcription regulation constitute the largest category. Four of them, which encode laccase, F-box family protein, SAC3/GANP family protein, and nadph-cytochrome P450 oxydoreductase, were validated using 5'-RACE. Conclusions: Our results indicate specific miRNAs are tightly regulated by salinity in shoots and/or roots of S. europaea, which play important roles in salt adaptation of this euhalophyte. The S. europaea salt-responsive miRNAs and miRNAs that target transcription factors, nucleotide binding site-leucine-rich repeat proteins and enzymes involved in lignin biosynthesis as well as carbon and nitrogen metabolism may be applied in genetic engineering of crops with higher stress tolerance, and genetic modification of biofuel crops with higher biomass and regulatable lignin biosynthesis.

Dataset Information

Integrated analysis of transcriptomics and proteomics approach the importance of ABA-dependent pathways in Mesembryanthemum Crystallinum leaf during the salt induced C 3 to CAM transition

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