Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Binding partners of Annexin A2 in MDA-MB-231 cells

ABSTRACT: A mass-spec of proteins found bound to Annexin A2 following a immunoprecipitation of Annexin A2 from cell lysate of MDAMB231 cells plated 1) on plastic or 2) on collagen-1

INSTRUMENT(S): QSTAR XL

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Patrick Kiely Amira Mahdi

PROVIDER: MSV000093084 | MassIVE | Wed Oct 11 09:37:00 BST 2023

REPOSITORIES: MassIVE

ACCESS DATA

Json Xml

Similar Datasets

HNRPA2B1 HITS-CLIP

Project description:Expression, crosslinking and immunoprecipitation of tagged HNRPA2B1 (variant A2) to identify bound RNA sequences

2012-02-28 | GSE35799 | GEO

Proteomic identification of IqgC binding partners

Project description:Putative binding partners of IQGAP-related protein IqgC were identified by LC-MS/MS. Recombinant GST-tagged IqgC was purified from E. coli and immobilised to glutathione agarose. Interactors from cell lysate of D discoideum AX2 cells were applied to prepared affinity resin and bound proteins were identified by LC-MS/MS.

2018-12-21 | PXD006311 | Pride

the RNA binding property of FSCN1

Project description:To investigate the RNA binding property of FSCN1 in ESCC, RNA immunoprecipitation (RIP) was performed using an FSCN1 monoclonal antibody in ECA-109 cell lysate

2022-03-05 | GSE197621 | GEO

HNRPA2B1 HITS-CLIP

Project description:Expression, crosslinking and immunoprecipitation of tagged HNRPA2B1 (variant A2) to identify bound RNA sequences RNA molecules that are immunoprecipitated are sequenced followed purification under denaturing conditions

2012-02-28 | E-GEOD-35799 | biostudies-arrayexpress

SMAD3 and SOX4 binding profiling in human breast cell lines

Project description:In order to identify genes co-bound by SOX4 and SMAD3 in the context of breast cancer, different breast cell lines (HMLEs, MDA-MB-231 or HCC-1954) were used. Due to low endogenous expression levels for SOX4 in HMLEs in untreated conditions, doxycycline-dependent SOX4 overexpression was obtained by transducing HMLE cells with pIINDUCER21-SOX4 vector..Cells were plated and SOX4 and SMAD3 chromatine-immunoprecipitation was performed in untreated conditions, TGF-beta (2.5ng/ml), doxycycline (0.5ug/ml) or both as indicated. Genome-wide binding sites for SOX4 and SMAD3 was identified in HMLEs, MAD-MB-231 and HCC-1954 cells.

2018-06-11 | GSE104760 | GEO

Mechanical cues control mutant p53 stability through a Mevalonate/RhoA axis

Project description:To investigate the genes differentially expressed upon plating on top of matrixes with different stiffness, we compared the expression profiles of MDA-MB-231 breast cancer cells plated on a stiff substrate (plastic) with the same cells plated on a soft substrate (hydrogels 0.7 kPa). Keywords: expression profiling by array

2017-12-23 | GSE93529 | GEO

Gene expression of rat PSCs cultured on plastic, matrigel and collagen

Project description:Activated pancreatic stellate cells produce the fibrotic matrix in chronic pancreatitis and pancreatic cancer. In vitro protocols examining PSC biology have usually involved PSCs cultured on plastic, a non-physiological surface. However, PSCs cultured on physiological matrices e.g. MatrigelTM (normal basement membrane) and collagen (fibrotic pancreas), may have distinctly different behaviours compared to cells cultured on plastic. Therefore, we aimed to compare PSC gene expression after culture on plastic, MatrigelTM and collagen I.

2013-01-17 | GSE43496 | GEO

Colonization of the Ovary and Metastasis of Fallopian Tube Derived Cancer

Project description:This experiment was designed to compare changes in the transcriptome of fallopian tube epithelial cells plated on two-dimensional and three-dimensional collagen.

2018-12-28 | GSE112397 | GEO

Targeting endoplasmic reticulum-anchored CRTH2 binding to LARP6 ameliorates organ fibrosis

Project description:Excessive deposition of extracellular matrix, mainly collagen protein, is the hallmark of organ fibrosis. The molecular mechanisms of the regulation of fibrotic protein biosynthesis are unclear. Here, we found that chemoattractant receptor homologous molecule expressed on TH2 cells (CRTH2), a cell membrane receptor for prostaglandin (PG) D2, is trafficked to the endoplasmic reticulum (ER) membrane in fibroblasts through Caveolin-1. CRTH2 anchored in the ER with bound collagen mRNA recognition motif (RRM) of La ribonucleoprotein domain family member 6 (LARP6) and promoted the degradation of collagen mRNA in fibroblasts. CRTH2 deficiency increased collagen biosynthesis in fibroblasts and exacerbated injury-induced organ fibrosis in mice, which was rescued by LARP6 depletion. Administration of CRTH2 N-terminal peptide reduced collagen production in fibroblasts by binding to LARP6. Similar to CRTH2, Bumetanide structurally bound to the LARP6 RRM domain, suppressed collagen biosynthesis in fibroblasts and alleviated bleomycin-triggered pulmonary fibrosis in mice. The findings reveal a novel anti-fibrotic function of CRTH2 in the ER membrane via interaction with LARP6, which may represent a therapeutic target for fibrotic diseases.

2021-04-25 | PXD025610 | iProX

The interacting partners of Fcer1g

Project description:To investigated the binding partners of Fcer1g to further explore its mediated signaling pathways, we utilized macrophage cell line RAW264.7 cells. After exogenously expressing Fcer1g-FLAG proteins, we performed immunoprecipitation-mass spectrometry (IP-MS) assay using FLAG antibodies to analyze the cell lysate.

2024-02-07 | PXD049222 | iProX

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data