Pulsed SILAC study of cell lines following PPIA knockdown

ABSTRACT: Mass spectrometry was performed with an Orbitrap Fusion Tribrid mass spectrometer (Thermo Scientific) interfaced with an UltiMate 3000 Binary RSLCnano System (Dionex). Proteome Discoverer v.1.4 (Thermo Scientific) with SEQUEST HT search engines was used for the spectra-preprocessing and HCD MS2 spectra were used for peptide identification and quantitation based on TMT reporter ions. Pulsed SILAC study of HEK293T cells and HeLa cells after knockdown (KD) of PPIA.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Andre Catic  

PROVIDER: MSV000093125 | MassIVE | Mon Oct 16 16:49:00 BST 2023

SECONDARY ACCESSION(S): PXD046245

REPOSITORIES: MassIVE