Label free proteomics of the IPF Lung

ABSTRACT: Idiopathic pulmonary fibrosis is a debilitating disease leading ultimately to death without existing treatment. Here we profiled the proteome of 30 IPF cores and 10 control cores. The goal was to validate findings of snRNA-seq and new informatics means to mine the data (UNAGI) and evaluate in silico drugs that could present efficiency against IPF. The samples were extracted using the MPLEx method, peptides were reduced, alkylated, and digested with trypsin and 5 ul of 0.1 ug/ul were injected on LC-MS/MS on a Lumos instrument. The analysis was performed in DDA label free mode.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Geremy Clair  

PROVIDER: MSV000093129 | MassIVE | Mon Oct 16 20:57:00 BST 2023

REPOSITORIES: MassIVE