Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Mitochondrial RNA Polymerase Post-translational Modifications

ABSTRACT: Proteomics data set of mitochondrial RNA polymerase (POLRMT) immunopurified from HeLa cells.

INSTRUMENT(S): 6545 Q-TOF LC/MS

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Kristin Dittenhafer-Reed

PROVIDER: MSV000093204 | MassIVE | Thu Oct 26 12:41:00 BST 2023

SECONDARY ACCESSION(S): PXD046430

REPOSITORIES: MassIVE

ACCESS DATA

Json Xml

Similar Datasets

Mitochondrial ribosomal protein L12 (MRPL12) post-translational modifications

Project description:Analysis of post-translational modifications on mitochondrial protein L12 (MRPL12). MRPL12 was overexpressed in HeLa or HEK293 cells, immunoprecipitated, and subject to bottom-up proteomic analysis on an Agilent 6545 LC-qTOF.

2023-11-08 | MSV000093335 | MassIVE

Numerous post-translational modifications of RNA polymerase II subunit Rpb4 link transcription to post-transcriptional mechanisms

Project description:Rpb4/7 binds RNA Polymerase II (Pol II) transcripts co-transcriptionally and accompanies them throughout their lives. By virtue of its capacity to interact with key regulators (e.g., Pol II, eIF3, Pat1) both temporarily and spatially, Rpb4/7 regulates the major stages of the mRNA lifecycle. Here we show that Rpb4/7 can undergo over 100 combinations of post-translational modifications (PTMs). Remarkably, the Rpb4/7 PTMs repertoire changes as the mRNA/Rpb4/7 complex progresses from one stage to the next. A mutagenesis approach in residues that undergo PTMs suggests that temporal Rpb4 PTMs regulate its interactions with key regulators of gene expression that control transcriptional and post-transcriptional stages. Moreover, one mutant type specifically affects mRNA synthesis despite its normal association with Pol II, whereas the other affects both mRNA synthesis and decay; both types disrupt the balance between mRNA synthesis and decay (‘mRNA buffering’) and the cell’s capacity to respond to the environment. Taken together, we propose that temporal Rpb4/7 PTMs are involved in cross talks among the various stages of the mRNA lifecycle.

2020-09-25 | PXD020511 | Pride

Post-translational modifications of mitochondrial aldehyde dehydrogenase and biomedical implications.

Project description: Not available

| S-EPMC3177986 | biostudies-literature

Comparison of mitochondrial transcription errors in wild type and mutant mitochondrial RNA polymerase overexpression flies

Project description:To determine the error rate of mitochondrial transcription, we ananlyzed 33 and 37 million reads respectively for wild type (WT) and mutant (E423P) mitochondrial RNA polymerase (POLRMT) overexpression flies and found that the error frequency of mitochondrial transcripts were over 5 fold higher in E423P flies than that of WT. To gain more insight into the molecular mechanisms that drive the error rate of transcription by POLRMT, we examined its distribution of errors along the mitochondrial genome. We also evaluated mitochondrial RNA processing by quantifying the frequency of a single read spanning two adjacent genes. There was no significant increase of unprocessed RNAs in E423P than that of WT. These observations concluded that overexpression of E423P POLRMT in adult flies leads to a statistically significant increase of mitochondrial transcripts errors.

2020-07-14 | GSE154310 | GEO

Post-translational modifications of microtubules.

Project description: Not available

| S-EPMC2951466 | biostudies-literature

Sirtuin Oxidative Post-translational Modifications.

Project description: Not available

| S-EPMC8652059 | biostudies-literature

Comparison of mitochondrial tRNA abundance profiling in wild type and mutant mitochondrial RNA polymerase overexpression flies.

Project description:To investigate differential mitochondrial tRNA gene expression in wild type (WT) and mutant (E423P) mitochondrial RNA polymerase (POLRMT) overexpression flies, we performed Drsophila mitochondrial tRNA-seq according to the Hydro-tRNAseq method. tRNA-seq reads were subjected to 3' - adapter filtering , 3' - adapter trimming and quality control. The tRNAs expression levels were measured by tag count. For each tRNA sequence-based profile, the mapped reads number used to estimate the expression level of each tRNA. The tRNAs expression profiling was calculated based on uniquely mapped reads and including mapped reads, respectively. We found no difference in the relative abundance of mitochondrial individual tRNAs between WT and E423P overexpression flies.

2021-01-06 | GSE164324 | GEO

Comparison of mitochondrial unprocessed RNA frequency in wild type and mutant mitochondrial RNA polymerase overexpression flies.

Project description:Comparison of mitochondrial unprocessed RNA frequency in wild type and mutant mitochondrial RNA polymerase overexpression flies.

| PRJNA791921 | ENA

Post-translational protein modifications in schizophrenia.

Project description: Not available

| S-EPMC7051976 | biostudies-literature

Lysine post-translational modifications of collagen.

Project description: Not available

| S-EPMC3499978 | biostudies-literature