The modified RNA base acp3U is an attachment site for N-glycans in glycoRNA

ABSTRACT: GlycoRNA consists of RNAs modified with secretory N-glycans that are presented on the cell surface. While previous work supported a covalent linkage between RNA and glycans, the direct chemical nature of the RNA-glycan connection was not described. Here we develop a sensitive and scalable protocol to detect and characterize native glycoRNAs. Leveraging periodate oxidation and aldehyde ligation (rPAL) and Sequential Window Acquisition of all Theoretical Mass Spectra (SWATH-MS), we identified the modified RNA base 3-(3-amino-3-carboxypropyl)uridine (acp3U) as a site of attachment of N-glycans in glycoRNA. rPAL offers sensitivity and robustness as an approach for characterizing direct glycan-RNA linkages occurring in cells, and its flexibility will enable further exploration of glycoRNA biology.

INSTRUMENT(S): ZenoTOF 7600

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Ryan A. Flynn   Benjamin A. Garcia  

PROVIDER: MSV000093213 | MassIVE | Tue Oct 31 20:32:00 GMT 2023

REPOSITORIES: MassIVE